{"id":60,"date":"2015-10-21T11:18:58","date_gmt":"2015-10-21T10:18:58","guid":{"rendered":"http:\/\/blog.u-bourgogne.fr\/list-maps\/?page_id=60"},"modified":"2017-11-06T15:49:36","modified_gmt":"2017-11-06T14:49:36","slug":"miguel-villoria-recio","status":"publish","type":"page","link":"https:\/\/blog.ube.fr\/list-maps-fr\/jeunes-chercheurs\/miguel-villoria-recio\/","title":{"rendered":"ESR 3"},"content":{"rendered":"

PR\u00c9SENTATION DU PROJET INDIVIDUEL 3<\/strong><\/p>\n

Regulation of the virulon of L. monocytogenes<\/em> by carbohydrates<\/strong><\/p>\n

\"carte<\/a>

Host organisation: University of Copenhagen (UCPH) Secondments: University College Cork (UCC) and University of Burgundy (UB)<\/p><\/div>\n

ORGANISATION D’ACCUEIL ET SUPERVISEUR:
\n<\/b><\/strong><\/p>\n

Universit\u00e9 de Copenhague, Dr. Hanne INGMER (plus d’informations)<\/a><\/p>\n

OBJECTIFS:<\/b><\/strong><\/p>\n

Preliminary data suggest that host sugar-molecules have chitin-resembling moieties and that these are substrates for both bacterial and human chitinases. Considering that the L. monocytogenes <\/em>chitinases are important in both environmental and host adaptation, we wish to identify the host chitin-type molecules and determine the role of these molecules as well as of the listerial chitinases for attachment, invasion and long-term survival in host cells. To assess the environmental role of the L. monocytogenes<\/em> chitinases we will determine the regulation of chitinase expression and investigate the conditions at which they are expressed. The role of chitinases in adaptability of L. monocytogenes<\/em> will be determined by investigation of the respective mutants in growth and survival in the various environments and the ability of the mutants to survive and initiate growth after abrupt changes in environments.Regarding the critical role of carbohydrates in regulation, this project will use combinations of proteomic and transcriptomic approaches to dissect the intricate regulatory mechanisms controlling chitinase expression. This will be studied using transposon mutagenesis and site-directed mutagenesis.<\/p>\n

R\u00c9SULTATS ATTENDUS:<\/b><\/strong><\/p>\n

Global transcriptomic response to chitin. Determination of TSS. Identification of proteins critical for regulation of chitinase expression. Evaluation of the role of chitinases during infection. Contribution of chitinases to soil and plant adaptation<\/p>\n

SECONDMENTS:<\/b><\/strong><\/p>\n

Universit\u00e9 Coll\u00e8ge Cork (plus d’informations)<\/a> et Universit\u00e9 de Bourgogne (plus d’informations)<\/a><\/p>\n

DUREE DU RECRUTEMENT :<\/b><\/strong><\/p>\n

36 mois<\/p>\n

 <\/p>\n

PR\u00c9SENTATION DU CHERCHEUR\"Miguel<\/a><\/strong><\/p>\n

Miguel VILLORIA RECIO<\/strong><\/p>\n

PAYS D’ORIGINE: <\/b><\/strong>Espagne<\/p>\n

FORMATION :<\/b><\/strong><\/p>\n