{"id":58,"date":"2015-10-21T11:18:30","date_gmt":"2015-10-21T10:18:30","guid":{"rendered":"http:\/\/blog.u-bourgogne.fr\/list-maps\/?page_id=58"},"modified":"2017-11-06T15:36:06","modified_gmt":"2017-11-06T14:36:06","slug":"vanessa-las-heras","status":"publish","type":"page","link":"https:\/\/blog.ube.fr\/list-maps-fr\/jeunes-chercheurs\/vanessa-las-heras\/","title":{"rendered":"ESR 2"},"content":{"rendered":"

PR\u00c9SENTATION DU PROJET INDIVIDUEL 2<\/strong><\/p>\n

Stress pre-adaptation and virulence potential of L.monocytogenes<\/i> in the food matrix<\/strong><\/p>\n

\"carte<\/a>

Recruteur: Universit\u00e9 de Cork (UCC) Secondments: Universit\u00e9 de Bourgogne (UB) et GenXPro (GXP)<\/p><\/div>\n

ORGANISATION D’ACCUEIL ET SUPERVISEUR:
\n<\/b><\/strong><\/p>\n

Universit\u00e9 de Cork, Dr. Cormac GAHAN (plus de d\u00e9tails)<\/a><\/p>\n

OBJECTIFS:<\/b><\/strong><\/p>\n

The project will examine the effects of important food components (including glutamate, arginine and carnitine) upon virulence potential in L. monocytogenes<\/i>. The ESR will utilise the ECadhum transgenic mouse system as a means to study the influence of food constituents upon pathogenesis using the pathogen in suspension (+\/- specific food components) and also using special defined chow containing particular food components as additives (from Teklad\/Harlan custom diets). The ESR will utilise classical microbiological approaches as well as bioluminescent imaging of L. monocytogenes<\/i> to track the pathogen in this system (IVIS system luminescence imaging). The ESR will also use deep sequencing approaches to determine the effects of food constituents upon gene expression profiles in L. monocytogenes<\/i> with a particular focus upon gene systems known to be induced in vivo during growth in the GI tract and necessary for virulence (e.g. SigmaB, BSH, bile, ADI, OpuC, GAD etc).<\/p>\n

R\u00c9SULTATS ATTENDUS:<\/b><\/strong><\/p>\n

Global transcriptomic response to food matrix constituents . Determination of TSS. Identification of proteins critical for survival in this habitat.<\/p>\n

SECONDMENTS:<\/b><\/strong><\/p>\n

GenXPRO (plus de d\u00e9tails)<\/a> et l’Universit\u00e9 de Bourgogne (plus de d\u00e9tails)<\/a><\/p>\n

DUR\u00c9E DU RECRUTEMENT:<\/b><\/strong><\/p>\n

36 mois<\/p>\n

 <\/p>\n

PR\u00c9SENTATION DU CHERCHEUR<\/strong><\/p>\n

Vanessa LAS HERAS<\/strong><\/p>\n

\"vanessa\"<\/a>PAYS D’ORIGINE:\u00a0 <\/b><\/strong>Portugal<\/p>\n

FORMATION:<\/b><\/strong><\/p>\n